 
Genetic Characterization
of Bull Trout from the Walla Walla River Basin
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SE Washington
Fish Reports
Genetic Characterization
of Bull Trout from the Walla Walla River Basin
Todd W. Kassler, Molecular Genetics Laboratory and Glen Mendel, Fish
Management – SE WA, Washington Department of Fish and Wildlife
Abstract
A total of 25
collections of bull trout were analyzed from eight different collection
sites within the Walla Walla River Basin and two collection sites
in the Yakima River Basin. Adult bull trout were analyzed from five
sites and juvenile bull trout were analyzed from five sites. Sixteen
nuclear microsatellite DNA loci that are included in the standardized
suite of loci were used to examine the levels and patterns of genetic
variation. The multi-locus genotypes generated for these bull trout
were analyzed to determine population structure of the adult and juvenile
bull trout collections. Tests of population subdivision, factorial
correspondence analysis, and the neighbor-joining tree suggested the
five adult collections were significantly different from one another.
The five juvenile collections were also all significantly different
from one another. The Touchet River adult collection clustered with
the juvenile collections, but was significantly different from them
in the genotypic tests of differentiation. The collections of juvenile
bull trout from the North Fork Touchet, Wolf Fork and Burnt Fork were
significantly different from one another with most of the statistical
comparisons we employed, while bull trout from the Lewis and Spangler
Creek collections could not be differentiated from the other groups
and tended to overlap or group with bull trout from the North Fork
Touchet or Wolf Fork. Assignment tests were used to determine stock-of-origin
percentage of migratory adult bull trout that were collected at Dayton
Dam Trap. The highest percentage of migratory bull trout at the Dayton
Dam came from Wolf Fork (50.6%) and the N.F. Touchet River (39.0%),
while Lewis Creek and Spangler Creeks accounted for 5.2% each, and
no adult bull trout assigned to Burnt Fork.
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